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Figure 2. Effect of spermicidal dermaseptins on sperm acrosome and flagellar plasma membrane integrity. Motile sperm were preincubated in the presence of spermicide solution (EC100 = 25 μg/mL) of K4S4 and was double labelled with Fluorescein Isothiocyanate-conjugated-Pisum sativum Agglutinin for acrosome labelling (FITC/green) and mouse antitubulin antibody for the flagellum staining (CY3/red). The sperm heads exhibited a “dotted” green fluorescence attesting for the presence of an acrosome. At the flagellar level, we observed a loss of membrane integrity since the anti tubulin antibodies have reached their axonemal microtubule target [44]. Image published in: Zairi A et al. (2009) Image downloaded from an Open Access article in PubMed Central. Copyright © 2009 Amira Zairi et al. Larger Image Printer Friendly View

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