Xenbase Image ID: 124808

FIGURE 1. Expression and function of wild type SERT and of mutated versions that lack candidate N-terminal phosphorylation sites. A, representative confocal laser scanning microscopy images illustrating that the cellular expression of SERTS62A and SERTT81A is comparable with that of wild type SERT. All constructs are almost exclusively targeted to the plasma membrane (also see for the ratio staining of plasma membrane to cell interior in Table 2). HEK293 cells were transiently transfected with CFP-tagged transporter constructs; the cell membrane was visualized by trypan blue staining (26). B, saturation of [3H]5HT uptake by SERT and the mutants expressed in transiently transfected CAD cells. Nonspecific uptake was determined in the presence of 10 μm paroxetine (n = 3–6, each experiment performed in triplicate). Image published in: Sucic S et al. (2010) © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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