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Figure 8. . Effects of MTSET and MTSES on the I-V relationship and Erev of four cysteine mutants: G584C (A), T613C (B), S631C (C), and D609C (D). In all cases oocytes have been DTT treated. The recording conditions, voltage clamp protocol, and data analysis are the same as those described for Fig. 2. After control data (open circles) are obtained, the cells are exposed to MTSET (1 mM) or MTSES (1 mM) while the channels are activated by constant pulses from Vh −80 to 20 mV for 1 s once per 30 or 60 s till changes in currents reach a steady state. The MTS reagents are washed out for ≥10 min and then currents after MTS modification (closed circles) are recorded. Image published in: Liu J et al. (2002) Copyright © 2002, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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