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Fig. 4. Pbht knockout attempt. A. A double cross-over knockout strategy used to attempt a knockout of pbht and investigate its function; the locations of primers used for PCR analysis of the locus are indicated by arrows. Tg DHFR/TS, Toxoplasma gondii dihydrofolate reductase/thymidylate synthase. B. PCR analysis of the pbht locus in wild type (WT) and parasites transfected with the knockout construct; lane 1, detection of the wild-type locus 1.1 kb (primers Pb7 + Pb9); lane 2, integration detection 1.2 kb (primers p539 + Pb9); lane 3, episome detection 1.1 kb (primers p539 + Pb8).

Image published in: Slavic K et al. (2010)

Journal compilation © 2010 Blackwell Publishing. Creative Commons Attribution license

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