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XB-IMG-125446

Xenbase Image ID: 125446

FIGURE 4. Effects of LB3 and Eg5 on the length, poles, and MT flux of spindles assembled from sperm chromatin. A, images of spindles formed in mock-depleted and LB3-depleted egg extracts. LB3 depletion resulted in larger spindles and an increase in multipolar spindles. B, quantifications of spindle length, excluding half-spindles and monopolar spindles, in both mock-depleted and LB3-depleted extracts in the presence or absence of 15 μm Monastrol. Monastrol rescued the spindle length change caused by LB3 depletion. C, quantification of the percentage of bipolar spindles, excluding half-spindles and monopolar spindles, in mock-depleted extracts and LB3-depleted extracts in the presence or absence of 15 μm Monastrol. LB3 depletion caused a decrease in the percentage of bipolar spindles, which was corrected with the addition of 15 μm Monastrol. Approximately 35 spindles were analyzed per condition per experiment, and three experiments performed on different days were used for quantifications. Error bars, S.E. D, speckle images of sperm spindles. The gray image shows a frame of the speckled spindle. Speckles moving toward opposite spindle poles are labeled as green and red dots. E, speckle trajectories color-coded according to their average instantaneous speckle velocities shown on the histogram to the right (flux rate, 2.031; S.D., 0.488; speckles tracked, 4488). Scale bars, 10 μm.

Image published in: Goodman B et al. (2010)

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons Attribution-NonCommercial license

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