XB-IMG-169460
Xenbase Image ID: 169460
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Fig 1. Pictorial representation of the process to assemble a transcriptome.First, RNA was extracted from the cloacal gland and HiSeq Illumina reads were created. The sequence reads were parsed and filtered for quality and removal of adaptor sequences (blue). Next, de novo assembly was generated and the transcripts were filtered based upon BLAST hits and redundant contigs were removed (red). Reads were mapped back to contigs, genes were annotated, and gene ontology was applied using BLAST and BLAST2GO (green). Finally, an analysis of the assembly and the quantity and distribution of transcripts was performed. Image published in: Hall KW et al. (2016) © 2016 Hall et al. Creative Commons Attribution license Larger Image Printer Friendly View |