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Figure 4. Determination of the stalling sites of DNA replication. (A) Experimental strategy to clone the stalled intermediates. The arrows illustrate the primers used for PCR. The templates for tailing and PCR were the gel-purified relaxed DNA from the 20 min time point. (B) Sequencing data of the PCR products showing the position of stalling.

Image published in: Liao S et al. (2007)

© 2007 The Author(s). Creative Commons Attribution-NonCommercial license

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