Xenbase Image ID: 126527

Figure 6. Nucleosome disassembly by RSC and SWI/SNF is not enhanced by H3 tail acetylation. The rate at which one nucleosome is removed from the 601–603 dinucleosome was determined using conditions similar to that in Figure 5, except that the ATP concentration was increased to 55 µM and incubated at 25°C. The appearance of the second remodeled species (II) was followed by gel shift on a 4% native polyacrylamide gel for RSC (A), SWI/SNF (C), and ΔBr SWI/SNF (E). The concentration of remodeled species II formed by RSC (B), SWI/SNF (D), and ΔBr SWI/SNF (F) was plotted against time and fitted non-linearly to the Michaelis–Menten equation when using non-acetylated (H3) or H3 acetylated (H3Ac) dinucleosomes. Image published in: Chatterjee N et al. (2011) © The Author(s) 2011. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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