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Figure 2. Knockdown of Kif4 inhibits LPA-induced formation of Glu MTs in NIH3T3 fibroblasts.A, B) Glu MT and Tyr MT staining of LPA-stimulated NIH3T3 fibroblasts transfected with the indicated siRNAs. C) Quantification of the percent of siRNA-treated cells that scored positive for Glu MTs. Two different siRNAs targeting Kif4 (#1 and #2) gave similar results. n>100 cells; error bars, SEM from at least 5 experiments. D, E) Western blots of NIH3T3 fibroblasts treated with indicated siRNAs and blotted for the indicated proteins. Quantification of the bands revealed over 70% knockdown of the indicated kinesins. Bars: A, B, 20 μm.

Image published in: Morris EJ et al. (2014)

Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license

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