XB-IMG-129726
Xenbase Image ID: 129726
![]() |
Figure 2. Knockdown of Kif4 inhibits LPA-induced formation of Glu MTs in NIH3T3 fibroblasts.A, B) Glu MT and Tyr MT staining of LPA-stimulated NIH3T3 fibroblasts transfected with the indicated siRNAs. C) Quantification of the percent of siRNA-treated cells that scored positive for Glu MTs. Two different siRNAs targeting Kif4 (#1 and #2) gave similar results. n>100 cells; error bars, SEM from at least 5 experiments. D, E) Western blots of NIH3T3 fibroblasts treated with indicated siRNAs and blotted for the indicated proteins. Quantification of the bands revealed over 70% knockdown of the indicated kinesins. Bars: A, B, 20 μm. Image published in: Morris EJ et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license Larger Image Printer Friendly View |