Xenbase Image ID: 126249

Figure 1. CMP6 cDNA isolation. (A) Sequence alignment of DC066238. Amino acid (aa) alignment was deduced by querying the DC066238 amino acid sequence using GENETYX. The amino acid homology is indicated as follows: identical (asterisk), very similar (double dot) and similar (dot). (B) The strategy is shown for generating full-length cDNA. First strand cDNA (dotted) and PCR products (thick) are shown with black lines. CMP6: 5'-RACE and 3'-RACE products represent Round 1 and Round 2, respectively. 3'-RACE products are shown in panel (C) lane 1, and 5'-RACE PCR products in (D) lane 1. PCR products were electrophoresed in a 1% agarose gel. Black arrowheads on panels (C) and (D) indicate the 1,700 and 1,296 bp of target products of CMP6. Molecular weights are shown for 1 kb Plus size markers (G&P, Anyang, Korea). Image published in: Lee RM et al. (2011) Copyright © 2011 Korean Association for Laboratory Animal Science. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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