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Figure 6. Transporter activity of cysteine mutant with only intact disulfide bridge (Cys-249 and Cys-321 residues) decreases in the presence of DTT, TCEP and GSH.Wild type and mutant SNAT4 expressing oocytes were incubated with DTT (0–10 mM) (A), TCEP (0–10 mM) (B) and 10 mM GSH (D). [3H]-alanine uptake assay was performed. Under reducing conditions, L-alanine transport in mutant SNAT4 significantly decreased. (C) Mutant SNAT4 expressing oocytes were incubated with 1 mM TCEP in the presence and absence of 0.02% H2O2. Under oxidative conditions, L-alanine transport in mutant SNAT4 was significantly recovered. Data is presented as mean ± SEM, n = 3 (∼ 10 oocytes/sample). Treated versus untreated control of the mutant, ***, P<0.001.

Image published in: Padmanabhan Iyer R et al. (2013)

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