Xenbase Image ID: 127862

Figure 3. Upregulated expression requires injection of the microRNA into the nucleus.(a) Nuclear injections (black bars) of miRcxcr4 but not control let-7a (miRcontrol) with cytoplasmic injection of the CX and Renilla reporters rescues upregulated expression of the CX reporter unlike cytoplasmic injections of miRcxcr4, where modest repression is observed (gray bars); injections were performed with transcribed RNA as depicted above.(b) mRNA levels of the CX reporter normalized to Renilla reporter levels as analyzed by qRT-PCR with nuclear or cytoplasmic injections of the microRNA do not correlate with translation changes. The average values of three replicates with standard deviations as error bars are shown in a-b. Image published in: Truesdell SS et al. (2012) Copyright © 2012, Macmillan Publishers Limited. Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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