Xenbase Image ID: 124967

FIGURE 2. The TPX2270–289 NLS binds to a range of Xenopus and mouse importin-α constructs. A, lanes 1 and 4 are crude bacterial lysate expressing the importin-α isoform; lanes 2 and 5 are the pull-down using GST- TPX2270–289; and lanes 3 and 6 are the pull-down using GST alone. GST-TPX2270–289 was able to bind mouse ΔIBB-importin-α2 and Xenopus ΔIBB-importin-α1a specifically from crude bacterial lysates, whereas virtually no binding was seen to GST alone. B, GST-TPX2270–289 binds to a range of ΔIBB importin-α isoforms derived from either mouse or Xenopus. Image published in: Giesecke A and Stewart M (2010) © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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