XB-IMG-178485
Xenbase Image ID: 178485
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Figure 1. Barhl2 influences the establishment of the organizational plan of the Xenopus neurula. (A) RT-qPCR for Xenopus laevis barhl2 from at least 10 individual embryos stage (st)-7.5-10 embryos. Error bars correspond to biological replicates. (B) ISH using xbarhl2 as probe showing expression in the presumptive organizer territory. A half-sliced embryo at st 8.5 is shown animal side up, dorsal right. (C) Representative st-25 embryos injected as indicated. The length of the cement gland (CG, black arrow) was measured using ImageJ. A CG of 1.2 times the average size of its wt siblings is considered increased. A CG of 0.8 times the average size of its wt siblings, or absent, was considered decreased. (D) Quantification of C. (E) Quantification graph showing that Barhl2 rescues the MObarhl2-induced CG phenotype. Image published in: Sena E et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |