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Figure 4. Plx1 deficiency affects Cdc45 loading and Cdk2 activity in the presence of stalled replication forks. (A) Cdc45 and Mcm7 binding to chromatin isolated from extracts that were untreated (CTRL), Plx1 depleted (ΔPlx1) or Plx1 depleted and reconstituted with recombinant Plx1 (+Plx1). All extracts were supplemented with aphidicolin. (B) Nuclear Cdk2 activity measured by monitoring 32P incorporation in histone H1 after immunoprecipitating nuclear Cdk2 with anti-Xenopus Cdk2 antibodies from extracts that were untreated (CTRL), mock depleted (Mock) or Plx1 depleted (ΔPlx1) and treated with and without 50 μM aphidicolin (APH) or 5 mM caffeine (Caff), as indicated.

Image published in: Trenz K et al. (2008)

Copyright © 2008, European Molecular Biology Organization. Creative Commons Attribution-NonCommercial-NoDerivatives license

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