XB-IMG-75263
Xenbase Image ID: 75263
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FIG. 4. Ectopic retinal structures form after expression of pax6 in ventral blastomeres. (A) Transverse sections through stage-44 to -45 embryos in which pax6 was injected into the V1.2.1 blastomere. (A) DIC image of an inverted eye-like structure near the otic vesicle. Le, lens-like tissue; arrow, RPE-like tissue. (B) Extension of the retinal pigmented epithelium (left arrow) toward the brain and displaced photoreceptor (PH) cells within a pax6/GFP-expressing clone within the retina. (C) Ectopic photoreceptor cells (arrows) in an ectopic eye-like structure. (D) Retina appears duplicated, as indicated by the double-cup arrangement of photoreceptors (red) associated with an enlarged lens (le) that is expressing pax6 (green). (E, F) Transverse sections through stage-44 to -45 embryos in which pax6 and noggin mRNA were coinjected into the V1.1.1 blastomere. (E) Ectopic photoreceptor (PH) located in a circular, GFP-positive structure near the heart (ht). (F) Ectopic site of RPE (arrow) near a normal retina. Injection of noggin alone into V1.1.1 does not cause the formation of ectopic retinal tissue (Table 1). Image published in: Kenyon KL et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |