Xenbase Image ID: 125261

Figure 6. Cdc6 inhibits Cdc7-dependent phosphorylation of Mcm2–7 on chromatin during DNA replication. (A) Silver staining (upper) and autoradiography (lower) profiles of the kinase assay using recombinant proteins. Mouse Mcm2–4–6–7 complex was incubated at 30°C for 60 min without (lanes 7 and 8) or with human Cdc7–ASK (lanes 1–6) in the absence (lanes 7 and 8) or presence of GST–Cdc6 (160, 320, 480, 640, 800 and 800 nM for lanes 2, 3, 4, 5, 6 and 7, respectively). (B) Chromatin was isolated after 0-, 15-, 30- or 45-min incubation of sperm nuclei (3 ng DNA/µl) with extracts supplemented with buffer (none) or GST–Cdc6 (700 nM). Isolated chromatin was subjected to immunoblotting. Image published in: Kundu LR et al. (2010) © The Author(s) 2010. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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