XB-IMG-128241
Xenbase Image ID: 128241
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Figure 2. Cadmium stabilizes the loop-gate closed state of Cx32*43E1 N2E E109C hemichannels. (A) A segment of a continuous current trace evoked by a train of alternating voltage polarizations from −10 mV (10 s duration) to −70 mV (10 s duration) with different [Cd2+] indicated by the colored solid bars. (B) Expansion of steady-state current traces shown in A. Current reductions at −70 mV (upward, positive going current relaxations) reflect closure of loop-gates. Increase in currents at −10 mV (downward, negative going current relaxations) reflect opening of loop-gates. (C) Steady-state peak currents measured at −70 mV are plotted against [Cd2+]. Half-maximal current reduction is obtained at [Cd2+] ∼10 µM. (D) Plots of normalized fitted currents of steady-state current relaxations at −70 mV. The time constants of current relaxations are shortened as [Cd2+] is increased. Current relaxations were well fitted by standard exponential function with two terms in Clampfit 9.0. (E) Plots of normalized fitted currents of steady-state current relaxations at −10 mV. Current relaxations in 0 and 10 µM Cd2+ were well fitted by single exponential functions. Current relaxations obtained with 10-s voltage applications became linear in higher [Cd2+] and could not be fitted to an exponential function. Image published in: Kwon T et al. (2013) © 2013 Kwon et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |