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XB-IMG-188667

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FIGURE 4. Nav1.5 and Nav1.7 migrate as non‐covalent homodimers under native PAGE. (a) SDS‐urea‐PAGE of hNav1.5 and hNav1.7 in the presence or absence of the reducing agent DTT. (b) and (c) hrCN‐PAGE of hNav1.5and hNav1.7 with various detergents used to turn dimers into monomers. The indicated channel proteins were extracted from Xenopus laevis oocytes with digitonin (a, b) or one of the indicated detergents (c), resolved by hrCN‐PAGE, and the GFP tags were visualized by Typhoon fluorescence scanning. Protein migration is shown under native conditions and after partial denaturation (1‐h incubation with 0.1% LiDS at 37°C). Numbers in the right margins in (b) and (c) indicate the sequence‐calculated masses (protomers to tetramers) of the partially denatured hTrpV1‐GFP channel. Numbers in the left margin in (c) correspond to the sequence‐calculated masses of the hNav1.7‐GFP protomer and homodimer. NG310, lauryl maltose neopentyl glycol; GDN, glyco‐diosgenin; DIG, digitonin

Image published in: Rühlmann AH et al. (2020)

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