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Figure 7. The S-L dimer channel has two subconductance levels. (A) Raw amplitude histograms were constructed and fitted with sums of Gaussian components as previously described (Chapman et al., 1997). Current levels arising from transitions (as judged by their slope) were omitted. At all potentials studied, four Gaussians were required; in addition to the fully open and closed level there were two well-resolved sublevels (Sub1 and Sub2). (B) Conductances were estimated by plotting the single channel amplitudes versus membrane potential, resulting in values of 4 pS, 9 pS, and 15 pS for the Sub1, Sub2, and the fully open state, respectively. The 4 pS and 9 pS values are similar to the 15% and 37% subconductance levels reported previously for drk1-L at threshold (Chapman et al., 1997).

Image published in: Chapman ML and VanDongen AM (2005)

Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

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