XB-IMG-128226
Xenbase Image ID: 128226
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Figure 7. Solid-state NMR analysis of scNsp1- and Nup98-derived FG hydrogels. (A) 1D cross-polarization (CP) spectra probing very rigid parts of the scNsp1 FG hydrogel (black curve), a non-glycosylated Nup98 FG hydrogel (blue) and a O-GlcNAc-modified Nup98 gel (red). (B) INEPT spectra probing highly mobile regions of the same gels (colour coding as in A). (C) 2D CP spectra probing very rigid parts of the scNsp1 gel (black) and the non-glycosylated Nup98 FG hydrogel (blue). Indicated correlations reflect inter-atomic polarization transfer among aliphatic carbon positions (denoted by α-γ) for selected residue types. Resonance frequencies encode information about the identity of residues and their backbone conformations. (D) 2D direct excitation spectra comparing non-glycosylated (blue) and O-GlcNAc-modified (red) Nup98 FG hydrogels. Resonance peaks indicating β-strand conformation are labelled in green, random coil conformations are coloured in mustard yellow. Image published in: Labokha AA et al. (2013) Copyright © 2013, European Molecular Biology Organization. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |