Xenbase Image ID: 122851

Figure 2. Aurora B activity is required for kinetochore assembly in X. laevis CSF extracts. (A and B) Hesperadin was titrated into X. laevis CSF egg extracts. Extracts were supplemented with nocodazole and sperm nuclei and, after 45 min, fixed and processed for immunofluorescence (A, top). (A) Nuclei stained with antibodies to pH3S10 and the nuclear stain Hoechst33342. The intensity of pH3S10 staining relative to the nuclear stain is graphed (right). Graphs show mean plus standard deviation. (B) Nuclei immunostained for the kinetochore protein Zwilch. Kinetochore assembly is inhibited at 2 μM hesperadin. (C) Kinetochores were assembled in CSF extracts preincubated with either anti–Aurora A or B antibodies. Nuclei were stained with antibodies to dynactin subunit p150Glued. Bars, 5 μm. Image published in: Emanuele MJ et al. (2008) Copyright © 2008, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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