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Fig. 3. Michaelis-Menten constants (KM (mM)) (a) and maximal substrate-inducible currents (Imax (nA)) (b) of the GA derivatives as a function of membrane potential (Vc). hNaDC3-expressing oocytes were subsequently superfused with increasing substrate concentrations of 3OHGA: 0.1, 0.2, 0.5, 1, 2, and 5 mM; D- and L-2OHGA: 0.01, 0.05, 0.1, 0.5, and 1 mM in ORi at pH 7.5. Values were calculated using the SigmaPlot Systat program. Data were obtained at the end of a 10-s perfusion with the respective solution at the indicated Vc and were calculated from 7 oocytes of 3 donors for 3OHGA, for D-2OHGA from 3 oocytes from 3 donors, and for L-2OHGA from 5 oocytes from 4 donors.

Image published in: Brauburger K et al. (2011)

Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2011

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