XB-IMG-122254
Xenbase Image ID: 122254
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Figure 4. . Effect of MTS exposure on presteady-state relaxations of mutants. (A) Representative presteady-state relaxations recorded from an oocyte expressing the WT NaPi-IIa protein superfused in 100 mM Na+ (ND100) and 0 mM Na+ (ND0) for the voltage step protocol shown in the inset. (B) Main component of presteady-state relaxation for a noninjected oocyte (NI) and the same WT-expressing cell in A, resolved by applying a two-exponential fitting procedure (see materials and methods). The ON (step from â60 mV to test potential) and OFF (step from test potential to â60 mV) relaxations are shown for superfusion in ND100 (top and middle traces) and ND0 (bottom traces). (C) Main ON and OFF relaxations for a representative G134C-expressing oocyte before (âMTS) and after (+MTS) exposure to 1 mM MTSEA (1 mM) for 3 min, superfused in ND100 and ND0. (D) Main ON and OFF relaxations for a representative M533C-expressing oocyte before (âMTS) and after (+MTS) exposure to 1 mM MTSEA (1 mM) for 3 min, superfused in ND100 and ND0. Image published in: Ehnes C et al. (2004) Copyright © 2004, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |