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Figure 5. Barium alters the rate of MTSEA modification at residues required for modulation by divalent cations. Second-order rate constants were measured for three residues in the transition zone of the α7 AChR (E44C, N170C, and E172C). (A) Mean values for second-order rate constants for modification by MTSEA alone, MTSEA + ACh, and MTSEA + Ba2+. Ba2+ caused a significant decrease in MTSEA modification rates of both E44C and E172C, despite the loss of divalent cation-dependent modulation. Ba2+ did not have a significant effect on the modification rate of N170C, although ACh significantly increased the rate of modification of this residue. *Rate was significantly different from control (P < 0.05). ‡Rate was significantly different from that obtained in presence of ACh (P < 0.05). The plot of rate constant ratios (B) shows that the effect of Ba2+ on the rate of modification of E44C was significantly less than the effect of ACh. See Table 2 for summary including (n) for each condition. Image published in: McLaughlin JT et al. (2009) Copyright © 2009 McLaughlin et al; licensee BioMed Central Ltd. Creative Commons Attribution license Larger Image Printer Friendly View

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