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Figure 5. Control of the activities of purified and refolded chemokines by a standard chemotaxis assay.Dose-dependent chemokine-induced migration of Jurkat cells was evaluated using a transwell system. Varying concentrations (0 to 300 nM) of WT CXCL12 or tagged-CXCL12 (A) and WT CCL5 or tagged-CCL5 (B) were added to the lower chamber. The results are expressed as percentage of input cells that migrated to the lower chamber over 3 hours from 3 replicate wells per condition (data are means ± SD). For CCL5-LT-Strep averaged values are slightly lower than the control at 0 nM clearly indicating an absence of chemotactic activity induced by this chemokine. Image published in: Picciocchi A et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license Larger Image Printer Friendly View

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