Xenbase Image ID: 129614

Figure 6. Characterization of KRAB-domains in EPC fish cells.A: Example of indirect immunofluorescence analysis of the nuclear/cytoplasmic compartmentalization of Gal4-KRAB-NES proteins alone (a–c, Gal4-staining) or after co-transfection with human TRIM28 (d–f, Gal4-staining; g–i, TRIM28-staining; corresponding panes one below the other). Bar = 10 µm. B: Quantification of the nuclear/cytoplasmic compartmentalization experiments (5–10 independent experiments for each construct, between 105 and 301 cells counted per experiment) 48 hours after transfection. Exact numbers and statistical analysis are given in Table S3. C: Transcriptional repression potential of different KRAB domains measured by heterologous luciferase reporter assays using Gal4-KRAB effector constructs as in Figure 2. Filled bars represent experiments without exogenous human TRIM28, open bars those with co-transfection of TRIM28. The Gal4 result of each experiment in absence of TRIM28 was used as baseline and set to 1 and four independent experiments were run. Statistical significance of a 2-tailed paired T-test is indicated by one asterisk (p<0.05), two asterisks (p<0.01) and three asterisks (p<0.001). Image published in: Born N et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license Larger Image Printer Friendly View

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