XB-IMG-178632
Xenbase Image ID: 178632
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Figure S4: Detection of GAS2L2 in HNE cells from subject PCD-1367.
(A) Separation of proteins for GAS2L2 targeted proteomics analysis. Proteins were separated on a 4-12% gradient polyacrylamide gel as described in supplementary methods and stained with Coomassie blue to visualize proteins. The gel was cut into the boxed sections for analysis by mass spectrometry.
(B) Total RNA was isolated from cultured control HBE cells, control HNE cells, and PCD HNE cells (PCD1367). RT-PCR and corresponding normalization confirmed decreased expression of GAS2L2 in PCD HNE cells. Image published in: Bustamante-Marin XM et al. (2019) Copyright © 2019. The Author(s) Larger Image Printer Friendly View |