Xenbase Image ID: 129567

Figure 1. Knocking down XMAP215 leads to reduced axon outgrowth due to increased rates of axon retraction. (A) Western blot showing knockdown of XMAP215 with increasing levels of morpholino oligonucleotide (MO). (B-C) Confocal images of whole-mount embryo immunostaining for acetylated tubulin shows peripheral axon outgrowth at two days post-fertilization. (D-E) Quantification of axon outgrowth in culture after XMAP215 knockdown (KD). n = explant number (D), axon number (E). (F-I) Phase contrast images of axons from control or XMAP215 KD neural explants (*) after 24 hours of culturing. (J-K) Timelapse montage of representative axons. (See Additional file 1). (L-O) Quantification of axon outgrowth parameters from timelapse imaging, between 18 and 23 hours of culturing. Box-and-whisker plots indicate the mean (diamond), median, extrema and quartiles. *P < 0.05, **P < 0.01, ***P < 0.001 comparing KD with control. ns not significant. n = axon number. Bar is 50 μm for (B,C), 20 μm for (F-K). Image published in: Lowery LA et al. (2013) Copyright © 2013 Lowery et al. Creative Commons Attribution license Larger Image Printer Friendly View

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