Xenbase Image ID: 123668

Figure 1. Glycine substitution in the IS6-AID linker affects VDI. (A) Amino acid sequence of wild-type and mutant IS6-AID linker sequences from CaV1.2 and CaV2.1. SOPMA secondary structure prediction is indicated (Geourjon and Deleage, 1995). (B) Disruption of the IS6-AID linker accelerates CaV1.2 VDI. Representative normalized IBa traces at a test potential of +20 mV for the combination of the indicated CaV1.2 subunits and CaVβ2a. (C) ti300 values for data from B. Results of unpaired t tests or one-way ANOVA, as appropriate, are indicated as follows: N.S., P > 0.05, not significant; ***, P < 0.001. (D) G-V relationships in barium for the indicated combinations of CaV1.2 subunits and CaVβ2a. (E) Disruption of the IS6-AID linker accelerates CaV2.1 VDI. Representative normalized IBa traces of CaV2.1 wild-type and CaV2.1 GGG. Data in all figures are represented as mean ± SD. Image published in: Findeisen F and Minor DL (2009) © 2009 Findeisen and Minor. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page