Xenbase Image ID: 124548

Figure 3. Assessment of IRES activity present within the MMTV-5′-UTR in X. laevis oocytes. (A) Different concentrations (3.1, 6.25, 12.5 or 25 ng) of capped and polyadenylated RNA corresponding to dl ΔEMCV, dl 5′UTR-MMTV or dl HCV IRES vectors were microinjected into X. laevis oocyte as described in ‘Material and Methods’ section. Oocytes were harvested 24 h after the microinjection and processed. Renilla luciferase (RLuc) and Firefly luciferase (FLuc) activities were determined (in RLUs). The RLuc (left panel) and FLuc (right panel) activities for each RNA in each concentration are shown. Oocytes injected with 12.5 and 25 ng of RNA showed saturating RLuc values. RLU values for oocytes injected with 25 ng RNA were obtained by diluting samples (1/4 volumes) prior to measurement. Each value is the mean ± SEM from at least nine oocytes obtained from two different animals. (B) Capped and polyadenylated RNA corresponding to the dl ΔEMCV, dl 5′UTR-MMTV or dl HCV IRES vectors (6.25 ng) were microinjected into X. laevis oocyte as described in ‘Material and Methods’ section. Oocytes were harvested 3, 6, 12 or 24 h after the microinjection and processed. RLuc and FLuc activities were determined (in RLU). The RLuc (left panel) and FLuc (right panel) activities for each RNA in each concentration are shown. Each value is the mean ± SEM from at least three oocytes obtained from different animals. Image published in: Vallejos M et al. (2010) © The Author(s) 2009. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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