XB-IMG-133186
Xenbase Image ID: 133186
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Figure 6: CaD is required for CNC explants to spread and
segregate on FN matrix. (A) CNC explants were dissected from early
neurula embryos injected with control-MO or CaD-MO (10 ng) and
plated on FN. By 10 h, control-MO–expressing cells spread and
migrated extensively and segregated into three streams. However,
cells from CaD-MO–expressing explants failed to spread efficiently or
separate into streams. Coinjection of 100–200 pg of CaD largely
rescued the spread and segregation of the explants. Arrows with
numbers marked the segregated lobes. High-magnification
differential interference contrast imaging of the explants (B, 40×) and
fluorescence imaging of cells with membrane labeling (C, 20×) show
that whereas control-MO–expressing cells extended multiple
membrane protrusions, CaD-MO–expressing cells remained
unpolarized and were often rounded. (D) The cells were tracked for
2 h and trajectories plotted. Although the speed of migration was
largely unaffected, the directionality of migration was significantly
impaired by CaD-MO. Black traces indicate that the ratio of the final
distance to the entire route covered is >0.3, and red indicates that it
was <0.3. Image published in: Nie S et al. (2011) © 2011 Nie et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |