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Figure 3. Fits of Scheme 0+2′ to selected WT and V2 macroscopic ionic current time courses that reflect the final transitions. For WT, these include (A) tail currents at voltages between −93 and −193 mV (patch w448), and (B) time-dependent occupancies in the last closed state in the activation path CN-1, derived from reactivation time courses. Occupancies in CN-1 are indicated for hyperpolarizations to voltages Vh = −93, −153, and −193 mV as a function of the hyperpolarization duration th. Occupancy estimates were derived from the amplitude of the fast reactivation component, as described in a previous paper (Schoppa and Sigworth, 1998a), and reflect averages from one to four experiments. In the simulations of these data, we set αN-1 = 0 during the test pulse, or, effectively, βN-2 >> αN-1 at V ≤ −93 mV. Scheme 0+2′ also accounts for (C) V2's macroscopic ionic tail currents at voltages between −73 and +27 mV, and (D) V2's channel opening time courses after a prepulse to +7 mV. In D, the prepulse loads most channels into the last closed states, so that the test currents mostly reflect the kinetics of the final two transitions. All V2 data are from the same patch (v329). Image published in: Schoppa NE and Sigworth FJ (1998) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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