XB-IMG-154471
Xenbase Image ID: 154471
![]() |
igure 4. Single-Channel Recordings of Slack Mutants R455H and A945T in Xenopus Oocytes
(A and B) Representative on-cell single-channel trace recorded at 80 mV from a patch expressing Slack R455H (A) and the corresponding all-points amplitude histogram (B).
(C) Binomial distribution for five R455H channels based on the mean single-channel Po from four low-N R455H patches.
(D and E) Representative high-N trace (D) and all-points amplitude histogram (E) from a patch containing five Slack R455H channels.
(F) Cooperativity ratios (comparing P(k)obs for R455H versus WT Slack) were calculated from the means of three and five high-N patches for G455R and WT Slack, respectively. The ratio shows a significant deviation from the expected value of one for all five channel openings.
(G and H) Representative low-N recording (G) and corresponding all-points amplitude histogram (H) from a patch containing A945T channels 80 mV.
(I) Binomial distribution for six channels based on the mean single-channel Po in low-N A945T patches (n = 5 low-N patches).
(J and K) Representative high-N recording (J) and corresponding all-points amplitude histogram (K) from patches containing six A945T channels.
(L) Cooperativity ratio as calculated from the means of three and five high-N patches for A945T and WT Slack, respectively. Image published in: Kim GE et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |