Xenbase Image ID: 125969

Figure 3. Open state stabilization, strong and weak coupling. (A) Gating currents of ShakerIR-W434F-Y485A in response to a series of depolarizing pulses (50 ms) from a holding potential (HP) of −90 mV to voltages between −120 and 60 mV in steps of 10 mV. (right) Corresponding QV relations for holding at 60, 0, and −90 mV are shown. Only a very modest mode shift is observed (P > 0.2). (B) Ionic currents of ShakerIR-A359C-I384A in response to a series of depolarizing pulses (50 ms) from a holding potential of −90 mV to voltages between −120 and 60 mV in steps of 10 mV. (right) Corresponding QV relations for holding at 0 and −140 mV and the GV (Vhold = −140 mV) are shown. The QVs are significantly shifted (P < 0.0001). (C) Gating currents of ShakerIR-W434F-F484A in response to a series of depolarizing pulses (50 ms) from a holding potential of −90 mV to voltages between −120 and 60 mV in steps of 10 mV. (right) Corresponding QV relations for holding at 0 and −90 mV are shown. They are not significantly shifted to one another (P > 0.7). (A–C) Data are shown as mean ± SD. (D) Gating and ionic currents of ShakerIR-F484A in response to depolarizing pulses from −90 mV to voltages between −120 and 60 mV in steps of 10 mV. (E) Effect of 100 mM TEA (top) and change in reversal potential to −30 mV in the presence of TEA (bottom; V = −70 mV). Only ionic current is affected, whereas gating currents remain the same. Image published in: Haddad GA and Blunck R (2011) © 2011 Haddad and Blunck. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page