XB-IMG-123815
Xenbase Image ID: 123815
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Figure 1. Competitive repositioning of histone H3 mutations by Chd1. (A) A native polyacrylamide gel for a repositioning assay using wild-type and H3 H39A nucleosomes assembled on 54A0 fragments, 6.9 fmol Chd1 and incubation at 30°C for the indicated times. (B) Chd1 repositioned H3 H39A nucleosomes 1.9-fold ± 0.1 (mean ± standard error of the mean) faster than wild-type nucleosomes. The raw data from the repositioning assays are plotted as points, the average hyperbolic fit of the data is shown as a line. (C) The initial rate of Chd1 repositioning is plotted for the histone H3 mutations. The dashed line marks an initial rate of 1. The error bars represent the standard error of the mean. WT, wild-type. Image published in: Somers J and Owen-Hughes T (2009) © 2009 The Author(s). Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |