Xenbase Image ID: 117871

Figure 6. The addition of mAb 70.1 to the cell-free mitotic aster assembly system is more deleterious to mitotic aster assembly than the depletion of cytoplasmic dynein. The cell-free HeLa mitotic extract was depleted using either a preimmune antibody (A) or an Eg5-specific antibody (B–E). The Eg5-depleted samples were further treated by either the depletion of cytoplasmic dynein (C) or the addition of the dynein-specific (D) or control (E) antibodies. After the induction of mitotic aster assembly under these conditions, the samples were fixed and processed for immunofluorescence microscopy using antibodies specific for tubulin and NuMA as indicated. Bar, 10 μm. Image published in: Gaglio T et al. (1997) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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