XB-IMG-126365
Xenbase Image ID: 126365
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Figure 1. Oct1pS335 is enriched in M-phase HeLa cells.(A) Whole cell extracts were prepared from normal or nocodozole-arrested HeLa cells. 10% polyacrylamide gels were Western blotted using anti-Oct1pS335 or anti-pan-Oct1 (C-terminal) antibodies (Bethyl). Anti-GAPDH is shown as a loading control. (B) HeLa cell whole cell extracts were immunoprecipitated with anti-Oct1pS335 antibodies and Western blotted using pan-Ub or pan-Oct1 antibodies. H.C.â=âimmunoglobulin heavy chain. (C) Whole cell extracts from nocodozole-arrested HeLa cells were treated with calf intestinal alkaline phosphatase (CIP), or mock-treated. Western blots using anti-Oct1pS335 or anti-pan-Oct1 antibodies are shown. Image published in: Kang J et al. (2011) Kang et al. Creative Commons Attribution license Larger Image Printer Friendly View |