XB-IMG-123816
Xenbase Image ID: 123816
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Figure 2. Histone H3 mutations differentially affect RSC repositioning. (A) Native polyacrylamide gel displaying repositioning of wild-type and H3 R49A nucleosomes assembled on 54A18 fragments by 0.44 fmol RSC at 30°C for the indicated times. Asterisk denotes P position. (B) RSC repositioned H3 R49A nucleosomes 2.1-fold ± 0.1 faster than wild-type nucleosomes. The raw data of the repositioning assays are shown as points, the curved line represents the average hyperbolic equation fit. (C) The initial rates of RSC repositioning for the histone H3 mutations. The dashed line indicates an initial rate of 1. Error bars represent the standard error of the mean. WT, wild-type. Image published in: Somers J and Owen-Hughes T (2009) © 2009 The Author(s). Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |