XB-IMG-118996
Xenbase Image ID: 118996
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Figure 8. DCC phosphorylation on tyrosine 1418 is critical for Netrin-1–induced Rac1 activation. (A) COS-7 cells coexpressing DCC, Y1261F-, or Y1418F-DCC mutants together with Myc-Rac1 protein were or were not treated with Netrin-1 for 5 min. The GTP-loaded Rac1 were pulled down from the cell lysates using GST-Cdc42/Rac interactive binding domain-Pak1 fusion protein. The proteins from the pull-down and from the total cell lysate were analyzed by Western blot using anti-DCC antibodies and anti-Myc antibodies to detect both Rac1-GTP and the total Rac1. (B) Quantitative analysis of Rac1 activation by DCC and DCC tyrosine mutants after Netrin-1 treatment (n = 5). Error bars represent SD. Image published in: Meriane M et al. (2004) Copyright © 2004, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |