Xenbase Image ID: 121223

Figure 5. . PIP2 reactivated run-down SK channels in a representative inside-out patch. (A) After run-down in the Ca2+-free, ATP-free bath solution, SK channel activity was restored by application of 50 μM PIP2. (B) PIP2 antibodies (anti-PIP2, 20 μM) caused SK channel run-down in the presence of Mg-ATP. (C) After removal of PIP2 antibodies, SK channel activity was not restored by Mg-ATP. However, SK channel activity was observed following addition of reducing agent, 100 μM DL-dithiothreitol (DTT). C = channel-closed state. −Vpipette = −40 mV. Image published in: Lu M et al. (2002) Copyright © 2002, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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