XB-IMG-159599
Xenbase Image ID: 159599
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Fig. 2. Summary of PCR results. The relative sizes and positions of clones obtained by PCR gene walking are summarized above. Gene walking allowed us to obtain 2146 bp of novel sequence from Prph2 and 813 bp of novel sequence from RCO using genomic DNA templates prepared with MscI, EcoRV, and HincII restriction enzymes. Clones obtained by this method terminated with restriction enzyme half-sites, as indicated. Clones tested for promoter activity in transgenic X. laevis are indicated, as is the relative position of the large Prph2 fragment obtained by conventional PCR. Image published in: Moritz OL et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |