XB-IMG-124774
Xenbase Image ID: 124774
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Fig. 2. Knockdown mutation of AtALMT12 impairs stomatal responses and has a wilty phenotype. (A) Schematic of the AtALMT12 locus (At4g17970) showing T-DNA insertion sites and primer locations (arrowheads) for RT–PCR analysis. (B) RT–PCR analysis using primer set #1 (or β-tubulin primers). (C) One-week-old wild-type (WT) and atalmt12-1 plants were subjected to water withholding for a further 2 weeks. Photographs of representative plants from three independent replicates were taken from the side and top. (D) Dark-induced stomatal closure (n = 4 experiments). (E) Calcium-induced stomatal closure (n = 4 experiments). (F) ABA-induced stomatal closure (n = 10 experiments for 0, 1 and 10 μM ABA; n = 4 experiments for 50 μM ABA). For D–F, 20 stomata were measured for each genotype in each experiment, and symbols or bars plot the mean ± SEM. (G) Light-induced stomatal opening in the atalmt12-1 mutant (n = 5 experiments). (H) Water loss of detached leaves (n = 3). Symbols plot the mean ± SEM. (I) Stomatal density. Plants were grown under short days (8L:16D) for 3 months or long days (16L:8D) for 3 weeks. Data are presented as the mean ± SD (n = 5 leaves). Differences from WT values were significant at *P < 0.05 and **P < 0.01, respectively. Larger Image Printer Friendly View |