Xenbase Image ID: 124013

Figure 7. Synaptic depression induced by postsynaptic secretion of endogenous pro-BDNF, which activates presynaptic p75NTR. (A) Down-regulation of pro-BDNF by BDNF morpholino. Xenopus BDNF–specific morpholino (Morph.) and scrambled (Scrm.) analogues were introduced by embryo injection. Neural tubes were dissected, and proteins were extracted. Western blots were performed using anti-BDNF antibodies. The blot was reprobed with an antitubulin antibody for loading controls. Note a reduction of pro-BDNF expression in BDNF morpholino–injected but not in uninjected (control [Ctl.]) or scrambled morpholino–injected embryos (left). Quantitative analysis of pro-BDNF expression in four independent experiments (right) is shown. The numbers in the bars indicate the number of Western blots performed. (B) Effect of presynaptic (N+) or postsynaptic (M+) expression of BDNF morpholino on depolarization-induced depression. (C) Effect of presynaptic or postsynaptic expression of p75NTR siRNA on depolarization-induced depression. (B and C) The numbers in the bars indicate the number of synapses recorded. (A–C) Error bars represent SEM. *, P < 0.05. In., inhibitor; stim., stimulation. Image published in: Yang F et al. (2009) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page