XB-IMG-175681
Xenbase Image ID: 175681
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Figure 5. Slow inactivation was not altered by either R1460W or R1460Q(A) Onset of slow inactivation showed identical kinetics between wild-type (WT) and mutant channels. Inset shows the voltage protocol to characterize the onset of slow inactivation by stepping to −10 mV for a variable duration (entry time), and then measuring the decline in relative loss current that fails to recover within 20 ms at −120 mV. (B) The rate of recovery from slow inactivation was comparable between WT and R1460 mutant channels. Channels were slow inactivated by a 30-second step to −10 mV (inset), and the data show recovery as the relative increase in current after repolarizing to −80 mV for a variable duration (recovery time). (C) The voltage dependence of steady-state slow inactivation was indistinguishable between WT and R1460 mutant channels. Thirty-second conditioning pulses to various conditioning potentials (inset) were used to determine the voltage dependence of inactivation. Smooth curves show best fits to the data with V1/2 of −59.1 ± 1.9 mV for WT, −60.9 ± 2.8 mV for R1460Q, and −63.4 ± 2.6 mV for R1460W. The number of cells for the mean values shown in the data was 5–8 for WT and both R1460 mutant channels. Image published in: Elia N et al. (2019) Copyright © 2019 The Author(s). Creative Commons Attribution license Larger Image Printer Friendly View |