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XB-IMG-198434

Xenbase Image ID: 198434


Figure 4. Xenopus RPE and photoreceptor marker expression following tbc1d32 knockdown. (A) Diagram of the experimental design. Whole mount (B) or retinal sections (C) following in situ hybridization against mitf or ihh, respectively, on embryos injected with control Mo or tbc1d32 Mo1. Scatterplots represent the quantification of the integrated density of the staining per eye relative to control Mo; each dot corresponds to 1 eye or 1 section, respectively. (D) Rho and SM opsin immunolabeling on retinal sections of embryos injected with control Mo or embryos injected with 2 doses of tbc1d32 Mo1 (10 or 15 ng). Lower panels, enlargement of the areas indicated by white dashed boxes in the upper panels. The bar plot represents the proportion of eyes with altered staining of Rho and SM opsin for each condition. The number of eyes analyzed per condition is indicated in each bar. Rho, rhodopsin; SM opsin, short and middle wavelength cone opsin. (E) Upper panels, whole-mount in situ hybridization against rhodopsin in embryos injected with control Mo or tbc1d32 Mo1. Lower panels, transverse retinal sections of control and morphant embryos. The scatterplots represent the quantification of the integrated density of rhodopsin staining relative to control Mo; each dot corresponds to 1 eye (left) or 1 section (right). For all scatterplots, data are represented as mean SEM. **P < 0.01; ***P < 0.001; ****P < 0.0001; Fishers exact test (D); 2-tailed Mann-Whitney test (B, C, and E). Scale bars = 400 m for whole-mount embryos and 40 m for sections.

Image published in: Bocquet B et al. (2023)

Copyright © 2023. Image reproduced with permission of the Publisher.

GeneSynonymsSpeciesStage(s)Tissue
mitf.LMITF-A, mitf-a, mitf-b, mitfaX. laevisThroughout NF stage 29 and 30eye
retinal pigmented epithelium
ihh.Lbanded hedgehog, bhh, indian hedgehog, X-BHH, X-hh, XbhhX. laevisThroughout NF stage 35 and 36eye
retinal pigmented epithelium
rho.Lopn2, rh1, rhodopsin, rp4, xrhoX. laevisThroughout NF stage 35 and 36retina
eye
photoreceptor layer
eye photoreceptor cell
retinal rod cell

Image source: Published

Experiment + Assay Source Phenotypes and Disease
tbc1d32 MO3 + NF St 3 + NF St 29-30 in situ hybridization
Disease
retinitis pigmentosa
Expression Phenotype
decreased amount mitf.L expression in retinal pigmented epithelium
tbc1d32 MO3 + NF St 3 + NF St 29-30 whole-mount microscopy
Disease
retinitis pigmentosa
Expression Phenotype
decreased amount mitf.L expression in retinal pigmented epithelium
tbc1d32 MO3 + NF St 3 + NF St 29-30 whole-mount microscopy
Disease
retinitis pigmentosa
Expression Phenotype
decreased amount mitf.L expression in retinal pigmented epithelium
tbc1d32 MO3 + NF St 3 + NF St 35-36 in situ hybridization
Disease
retinitis pigmentosa
Expression Phenotype
decreased amount ihh.L expression in retinal pigmented epithelium
tbc1d32 MO3 + NF St 3 + NF St 40 immunohistochemistry
Disease
retinitis pigmentosa
Expression Phenotype
decreased amount opn1sw.L expression in retina
decreased amount opn1sw.L expression in retinal cone cell
decreased amount rho.L expression in retina
decreased amount rho.L expression in retinal cone cell

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