XB-IMG-125708
Xenbase Image ID: 125708
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Figure 6. Regulatory potential of AmphiVent1 and Xvent-2B promoters in early developing medaka embryo.Transient expression of EGFP in medaka embryos injected with p817-AmphiVent1 (A-A′, D-E′) and p817-Xvent-2B (B-B′, F-G′) constructs examined under bright field (A′, B′, D′, E′, F′, G′) and bright field merged with fluorescence (A, B, D, E, F, G). (A-B′) EGFP expression driven by AmphiVent1 or Xvent-2B promoter at early gastrula stage. (D-G′) EGFP expression patterns in mid-gastrula stage medaka embryos injected with p817-AmphiVent1 (D-E′) and p817-Xvent-2B (F-G′); dorsal (D-D′, F-F′) and lateral (E-E′, G-G′) views show EGFP fluorescence in the blastoderm around the most dorsal region of embryonic shield (Sh). (C, H) Schematic diagram of developing medaka embryo depicting mRNA expression pattern of early dorsal mesoderm markers Chordin (stage 13, C) [30] and Goosecoid (stage 15, H) [31]. Dashed line indicates the borders of the blastoderm. White arrowheads depict the most dorsal embryonic shield of the medaka embryo, where Chordin and Goosecoid are expressed. GR-germ ring, Sh-embryonic shield. Image published in: Kozmikova I et al. (2011) Image downloaded from an Open Access article in PubMed Central. This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Larger Image Printer Friendly View |