Xenbase Image ID: 123710

Figure 1. FGF signaling in early development.(A) is a Western blot showing levels of diphospo-ERK (dp-ERK) in whole embryos from cleavage stage 6 to late blastula stage 9.5 (3 to 8 hours pf at 23°C). GAPDH is a ubiquously expressed loading control. (B) shows whole mount immunohistochemistry for dp-ERK in blastula stage 9 embryos. In animal hemisphere view dorsal side is to the right. In lateral view the animal hemisphere is to the top and the dorsal side is to the right. In dorsal view the animal hemisphere is to the top. (C) is a Western blot comparing dp-ERK levels in control uninjected embryos and embryos injected with 4 ng dnFGFR4 mRNA from blastula stage 9 to early gastrula stage 10.25. (D) shows whole mount immunohistochemistry for dp-ERK at blastula stage 9 in a control uninjected embryo and an embryo injected with 4 ng dnFGFR4 embryo. Embryos are viewed from the animal hemisphere with dorsal side to the right. (E) is an RNAase protection analysis (RPA) showing the expression of Cdx4, MyoD, brachyury and ODC in control uninjected embryos and embryos injected with 4 ng dnFGFR4 mRNA from early gastrula stage 10 until stage 10.5. ODC is a ubiquioulsy expressed loading control. 10 µg of total RNA were used per hybridization. (F) is a Western blot showing dp-ERK levels in control uninjected embryos, embryos injected with 4 ng dnFGFR1 mRNA and embryos injected with 4 ng dnFGFR4 mRNA at early gastrula stage 10.5. The embryos are siblings to one set of the three biological replicates used for the microarray analysis. (G) is an RPA showing expression of Cdx4, MyoD and brachyury in control uninjected embryos, embryos injected with 4 ng dnFGFR1 mRNA and embryos injected with 4 ng dnFGFR4 mRNA at early gastrula stage 10.5. The embryos are siblings to one set of the three biological replicates used for the microarray analysis. (H) shows whole mount in situ hybridizations for Cdx4, MyoD and brachyury in control uninjected embryos , embryos injected with 4 ng dnFGFR1 mRNA and embryos injected with 4 ng dnFGFR4 mRNA at early gastrula stage 10.5. The embryos are siblings to one set of the three biological replicates used for the microarray analysis. Image published in: Branney PA et al. (2009) Branney et al. Creative Commons Attribution license Larger Image Printer Friendly View

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