Xenbase Image ID: 128062

Figure 3. Effects of inhibitors of signaling molecules on the TaAQP7 transcripts under PEG and ABA treatments.A: Effects of pretreatment with inhibitors of ABA biosynthesis and scavenger H2O2 on the expression of TaAQP7 in the leaves of wheat seedlings exposed to PEG. The plants were pretreated with distilled water, 1 mM tungstate, and 5 mM DMTU for 2 h and 6 h respectively, and then exposed to 20% PEG6000 for 2 h and 6 h respectively. The treatment of tungstate or DMTU alone was also performed in the experiment. The plants treated with distilled water for 2 h or 6 h were used as control. B: Effects of pretreatment with scavenger of H2O2 on the expression of TaAQP7 in the leaves of wheat seedlings exposed to ABA. The plants were pretreated with distilled water, and 5 mM DMTU for 2 h and 6 h respectively, and then exposed to100 µM ABA for 2 h and 6 h respectively. The treatment of DMTU alone was also performed in the experiment. The plants treated with distilled water for 2 h or 6 h were used as control. The y-axis represents the relative fold difference in mRNA level calculated using the 2–ΔΔCt formula with TaActin as internal control. The mRNA fold difference was relative to that of distilled water treated samples used as calibrator. Vertical bars indicate ±SE of four replicates on one sample. Asterisks indicate significant difference between the expression of TaAQP7 under different treatment (*p<0.05; **p<0.01). When no bar is shown, the deviation is smaller than the symbol. Three biological experiments were performed, which produced similar results. Image published in: Zhou S et al. (2012) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license Larger Image Printer Friendly View

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