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Figure 7. Shortening of the first cycle period significantly reduces embryo viability, and cycloheximide rescues viability.(A–C) Application of PD0166285 during the first cycle causes a loss of viability, whereas later treatment does not. (A) Changes in the length of the first cycle in response to two concentrations of PD0166285. (B) Kaplan–Meier survival curves. (C) Survival at 44 h postfertilization. The data in (A) and (C) are from four experiments, whereas the data in (B) are from one representative experiment. (D–F) Cycloheximide (CHX, 0.25 µg/mL) partially rescues the effects of PD0166285 (30 µM) on viability. (D) Changes in the length of the first cycle in response to PD0166285 ± CHX. (E) Kaplan–Meier survival curves. (F) Survival at 44 h postfertilization. The data in (D) and (F) are from four experiments, whereas the data in (E) are from one representative experiment. (G) Photographs of drug-treated and control embryos at various times after fertilization. The embryos were placed in the same petri dish after the inhibitors were washed out at the completion of the first cycle. The arrows designate three PD-treated embryos that have discoordinated cell divisions as early as a few hours postfertilization; the other PD-treated embryos are grossly normal until the midblastula transition (bottom panel). The incubation temperature was 18° for the experiments in (A–F), and 23° for the experiment in (G).

Image published in: Tsai TY et al. (2014)

Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license

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